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Test

Internal Control

Abbreviations

Pathogen Category

Target Gene

Sample Type

Required Channels

Minimum Sensitivity (Copies/Ul)

Avian Influenza Virus Type H5,H7,H9 Multiplex

Exogenous

AIV-H5/H7/H9

RNA virus

HA gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

FAM; VIC; CY5; ROX

5

Avian Influenza Virus Type H5

Exogenous

AIV-H5

RNA virus

HA gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

FAM; VIC

5

Avian Influenza Virus Type H7

Exogenous

AIV-H7

RNA virus

HA gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

FAM; VIC

5

Avian Influenza Virus Type H9

Exogenous

AIV-H9

RNA virus

HA gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

FAM; VIC

5

Avian Influenza Virus

Exogenous

AIV-infA

RNA virus

M gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

FAM; VIC

5

Avian Leukosis Virus

Exogenous

ALV-U

RNA virus

P27 gene

Whole blood, plasma with white blood cells, serum, spleen, liver, kidney of suspected sick chickens

FAM; VIC

2.5

Avian Leukosis Virus J Subgroup

Exogenous

ALV-J

RNA virus

ENV gene

Whole blood, plasma with white blood cells, serum, spleen, liver, kidney of suspected sick chickens

FAM; VIC

2.5

Avian Leukosis Virus A,B Subgroup  Multiplex

Exogenous

ALV-A/B

RNA virus

gp85

Whole blood, plasma with white blood cells, serum, spleen, liver, kidney of suspected sick chickens

FAM; VIC

2.5

Avian Leukosis Virus A,B,C,D Subgroup Multiplex

Exogenous

ALV+A/B/E/J

RNA virus

gp85

Whole blood, plasma with white blood cells, serum, spleen, liver, kidney of suspected sick chickens

FAM; VIC

5

Newcastle Disease Virus

Exogenous

NDV-U

RNA virus

M gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

FAM; VIC

5

Avian Infectious Bronchitis Virus

Exogenous

IBV

RNA virus

N gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

FAM; VIC

2.5

Avian Infectious Laryngotracheitis Virus

Exogenous

ILTV

DNA virus

gB gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

FAM; VIC

2.5

Avian Infectious Bursal Disease Virus

Exogenous

IBDV

RNA virus

VP5 gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

FAM; VIC

2.5

Avian Reovirus

Exogenous

ARV

RNA virus

sigma C (S1)

Spleen tissue, secretions or excretions from the cloaca and trachea, synovial fluid from the tibiofemoral or tibio-tarsal joints.

FAM; VIC

5

Mycoplasma Gallisepticum

Exogenous

MG

DNA pathogens

pvpA

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

FAM; VIC

5

Mycoplasma Synovium

Exogenous

MS

DNA pathogens

VLHA

Internal inflammatory secretions from throat swabs, arthrogryposis and chest cysts

FAM; VIC

5

Mycoplasma Gallisepticum and Mycoplasma Synovium Multiplex

Exogenous

MG/MS

DNA pathogens

pvpA and VLHA

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung tissues; live birds to be examined: respiratory secretions, excretions; internal inflammatory secretions of joint cysts and thoracic cysts

FAM; VIC; ROX

5

Duck Plague Virus

Exogenous

DPV

DNA virus

UL6

Blood, digestive tract, lymphatic organs and organs, etc.

FAM; VIC

2.5

Chicken Salmonella Gallinarum

Exogenous

SG

DNA pathogens

glgC

Watery feces or suspected contaminants, etc.

FAM; VIC

5

Chicken Salmonella Gallinarum and Salmonella Pullorum Multiplex

Exogenous

SG/SP

DNA pathogens

glgC and RHS-family protein

Watery feces or suspected contaminants, etc.

FAM; VIC; ROX

5

Chicken Salmonella Enteritidis

Exogenous

SE

DNA pathogens

sdfI

Watery feces or suspected contaminants, etc.

FAM; VIC

5

Salmonella

Exogenous

Salm

DNA pathogens

InvA

Watery feces or suspected contaminants, etc.

FAM; VIC

5

Avian Adenovirus Type 4

Exogenous

FADV-4

DNA virus

Hexon protein

Tissue samples of liver, spleen and kidney from suspected infected deadavian

FAM; VIC

5

Avian Adenovirus Group I

Exogenous

FADV-I group

DNA virus

DNA polymerase gene

Tissue samples of liver, spleen and kidney from suspected infected deadavian

FAM; VIC

5

Marek's Disease Virus

Exogenous

MDV

RNA virus

meq

Feather marrow, liver, spleen, kidney and other organs, as well as lymphocytes and cell cultures

FAM; VIC

5

Duck Hepatitis A Virus Type 1

Exogenous

DHAV-1

RNA virus

polyprotein gene

Duck diseased liver tissue, duck embryo allantoic fluid or cell cultures inoculated with suspected diseased material

FAM; VIC

1

Duck Hepatitis A Virus Type 2

Exogenous

DHAV-2

RNA virus

ORF1a

Duck diseased liver tissue, duck embryo allantoic fluid or cell cultures inoculated with suspected diseased material

FAM; VIC

5

Duck Hepatitis A Virus Type 3

Exogenous

DHAV-3

RNA virus

VP1 gene

Duck diseased liver tissue, duck embryo allantoic fluid or cell cultures inoculated with suspected diseased material

FAM; VIC

2.5

Duck Hepatitis A Virus Type 1,2,3 Multiplex

Exogenous

DHAV-1+2+3

RNA virus

polyprotein gene+ORF1a+VP1

Duck diseased liver tissue, duck embryo allantoic fluid or cell cultures inoculated with suspected diseased material

FAM; VIC; CY5; ROX

5

Goose and Duck Reovirus

Exogenous

GoDuRV

RNA virus

S1 gene

Spleen of diseased geese and ducks

FAM; VIC

2.5

Gosling Plague Virus

Exogenous

GPV

DNA virus

VP2 gene

For dead or dying animals, samples of liver, pancreas, spleen, kidney and brain were taken aseptically. For live animals, collect whole blood. For animal products, collect viscera, tissues.

FAM; VIC

2.5

Avian Adenovirus Type 8b

Exogenous

FADV-8b

DNA virus

52/55 kDa protein

Tissue samples of liver, spleen and kidney from suspected infected deadavian

FAM; VIC

5

Avian Adenovirus Type 11

Exogenous

FADV-11

DNA virus

Hexon gene

Tissue samples of liver, spleen and kidney from suspected infected deadavian

FAM; VIC

5

Avian Adenovirus Type 4,8b,11 Multiplex

Exogenous

FADV4/8b/11

DNA virus

Hexon protein+52/55 kDa protein+hexon gene

Tissue samples of liver, spleen and kidney from suspected infected deadavian

FAM; VIC; CY5; ROX

5

Avian Paramyxovirus Type 1

Exogenous

APMV-1

RNA virus

F gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

FAM; VIC

2.5

Goose and Duck Parvovirus

Exogenous

GPV+MDPV

DNA virus

rep protein

Liver, spleen, pancreatic diseased tissue, embryonic urinary bladder fluid, etc.

FAM; VIC

2.5

Muscovy Duck Parvovirus

Exogenous

MDPV

DNA virus

rep protein

Liver, spleen, pancreatic diseased tissue, embryonic urinary bladder fluid, etc.

FAM; VIC

1

Duck Tambucu Virus

Exogenous

BYD, DTMUV

RNA virus

envelope protein

Ovaries, fallopian tubes, pancreas, brain and other tissues of sick ducks

FAM; VIC

2.5

Avian Adenovirus Group I, Infectious Bursal Disease Virus and Infectious Laryngotracheitis Virus

Exogenous

FADV-I/IBDV/ILTV

DNA, RNA virus

Specific conservative gene

Tissues such as laryngotrachea, brain, pectoral muscle, heart muscle, liver, spleen and kidney and lung; live birds to be examined: respiratory secretions, excreta, etc.

FAM; VIC; CY5; ROX

5

Goose Astrovirus

Exogenous

GoAstV

RNA virus

ORF1a

Tissue samples of liver, spleen and kidney suspected of infection

FAM; VIC

2.5

Reticuloendotheliosis Virus

Exogenous

REV

RNA virus

ENV-gp90 gene

Whole blood, plasma with white blood cells, serum, spleen, liver, kidney of suspected sick chickens

FAM; VIC

5

Avian Metapneumovirus

NoInternal Control

aMPV

RNA virus

N gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

SYBR Green

5

Egg Drop Syndrome Virus

Exogenous

EDSV

DNA virus

L3 23K gene

Cull suspected EDSV-infected chickens, take appropriate samples of oviducts and follicular membranes, and poor quality egg whites

FAM; VIC

2.5

Chicken Infectious Anemia Virus

Exogenous

CIAV

DNA virus

VP1 gene

Liver of dying chickens and culled chickens; blood of live chickens to be examined

FAM; VIC

2.5

Newcastle Disease Virus,Avian Influenza Type H9, and Avian Infectious Bronchitis Virus Multiplex

Exogenous

NDV/AIV-H9/IBV

RNA virus

HA gene/N gene/M gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

FAM; VIC; CY5; ROX

5

Avian Pneumovirus

Exogenous

APV

RNA virus

F gene

Blood, laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

FAM; VIC

5

Avian Encephalomyelitis Virus

Exogenous

AEV

RNA virus

VP1 gene

Serum, brain tissue, etc.

FAM; VIC

2.5

Newcastle Disease Virus and Avian Influenza Virus Type H9 Multiplex

Exogenous

NDV/AIV-H9

RNA virus

HA gene/N gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

FAM; VIC; ROX

5

Avian Hepatitis E Virus Multiplex

Exogenous

aHEV

RNA virus

Specific conservative gene

Diseased liver tissue, bile, blood, feces, etc.

FAM; VIC

5

Avian Adenovirus Group I, Mycoplasma Gallisepticum and Mycoplasma Gallisepticum Multiplex

NoInternal Control

FADV-I/MG/MS

DNA virus/bacteria

Specific conservative gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung tissues; live birds to be examined: respiratory secretions, excretions; internal inflammatory secretions of joint cysts and thoracic cysts

FAM; VIC; ROX

5

Reticuloendotheliosis Virus, Avian Reovirus , Avian Leukosis Virus and Avian Encephalomyelitis Virus Multiplex

NoInternal Control

REV/ARV/ALV/AEV

RNA virus

Specific conservative gene

Whole blood, plasma with white blood cells, serum, spleen, liver, kidney of suspected sick chickens, etc.

FAM; VIC; CY5; ROX

5

Avian Leukosis Virus A,B,C,D Subgroup Multiplex

NoInternal Control

ALV-ABCD

RNA virus

gp85

Whole blood, plasma with white blood cells, serum, spleen, liver, kidney of suspected sick chickens

FAM; VIC; CY5; ROX

5

Fowlpox Virus

Exogenous

APXV

DNA virus

Specific conservative gene

Lesioned tissue, and blood

Avian Influenza Virus Type H5(RT-PCR)

NoInternal Control

AIV-H5

RNA virus

HA gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

Agarose gel

50

Avian Influenza Virus Type H7(RT-PCR)

NoInternal Control

AIV-H7

RNA virus

HA gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

Agarose gel

50

Avian Influenza Virus Type H9(RT-PCR)

NoInternal Control

AIV-H9

RNA virus

HA gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

Agarose gel

500 TBD

Avian Influenza Virus(RT-PCR)

NoInternal Control

AIV-U

RNA virus

Polysaccharide protein gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

Agarose gel

50

Avian Infectious Bronchitis Virus(RT-PCR)

NoInternal Control

IBV

RNA virus

N gene

Laryngeal trachea, brain, thoracic muscle, heart muscle and lung and other tissues; live birds to be examined: respiratory secretions, excreta, etc.

Agarose gel

500 TBD

Avian Reovirus(RT-PCR)

NoInternal Control

ARV

RNA virus

S1 gene

Spleen tissue, secretions or excretions from the cloaca and trachea, synovial fluid from the tibiofemoral or tibio-tarsal joints.

Agarose gel

500 TBD

Avian Adenovirus Group I(PCR)

NoInternal Control

ARV

RNA virus

52K gene

Tissue samples of liver, spleen and kidney from suspected infected deadavian

Agarose gel

5