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Test

Internal Control

Abbreviations

Pathogen Category

Target Gene

Sample Type

Required Channels

Minimum Sensitivity (Copies/Ul)

Enterocytozoon Hepatopenaei

Exogenous

EHP

DNA pathogens

18S rRNA gene

1, Fertilized eggs, juvenile shrimp, intact individual shrimp, 2, juvenile to adult shrimp or adult shrimp: head and thorax with the eye stalk, intestinal tissue or feces removed. 3, bait, processed aquatic products and other samples

FAM; VIC

5

Vibrio Harveyi

Exogenous

VH

DNA pathogens

ToxR gene

Shrimp lesion tissue, parent shrimp, shrimp fry, processed aquatic products, etc.

FAM; VIC

2.5

White Spot Syndrome Virus

Exogenous

WSSV

DNA virus

ORF167 gene

Adult shrimp gills or epithelium, hepatopancreas, stomach, swimming foot or gait foot and other focal areas; fertilized eggs, juvenile shrimp, young shrimp, intact individuals. Bait, processed aquatic products and other samples.

FAM; VIC

2.5

Acute Hepatopancreatic Necrosis Syndromes Syndromes

Exogenous

EMS

DNA pathogens

PirAB gene

Adult shrimp gills or epithelium, hepatopancreas, stomach, swimming foot or gait foot and other focal areas; fertilized eggs, juvenile shrimp, young shrimp, intact individuals. Bait, processed aquatic products and other samples.

FAM; VIC

5

Shrimphemocyteiridescentvirus

Exogenous

SHIV, DIV1

DNA virus

ATP gene

Heart, muscle, subcutaneous tissue, gills, hepatopancreas and other tissues; fertilized eggs, juvenile shrimp, young shrimp intact individuals. Other samples such as bait and processed aquatic products.

FAM; VIC

5

Taurasyndrome Virus

Exogenous

TSV

RNA virus

VP1 gene

Larger shrimp: remove the eye stalk of the head thorax, gills, lymphatic organs, etc.; fertilized eggs, juvenile shrimp, young shrimp, intact individuals. Non-shrimp crustaceans refer to the method of sampling shrimp. Bait, processed aquatic products and other samples.

FAM; VIC

2.5

Siniperca Chuatsi Rhabdovirus

Exogenous

SRCV

RNA virus

N gene

Fish brain, liver, kidney, spleen, gills and other tissues; bait, processed aquatic products and other samples.

FAM; VIC

2.5

Large Mouth Bass Ulcerative Syndrome VirusVersion 1.0

Exogenous

LBUSV, LMBV

DNA virus

MCP gene

Fish brain, liver, kidney, spleen, gills and other tissues; bait, processed aquatic products and other samples.

FAM; VIC

5

Large Mouth Bass Ulcerative Syndrome VirusVersion 2.0

Exogenous

LBUSV, LMBV

DNA virus

MCP gene

Fish brain, liver, kidney, spleen, gills and other tissues; bait, processed aquatic products and other samples.

FAM; VIC

5

Infectious Spleen and Kidney Necrosis Virus

Exogenous

ISKNV

DNA virus

MCP gene

Fish brain, liver, kidney, spleen, gills and other tissues; bait, processed aquatic products and other samples.

FAM; VIC

2.5

Frog Virus Type 3

Exogenous

FV3

DNA virus

MCP gene

Fish brain, liver, kidney, spleen, gills and other tissues; bait, processed aquatic products and other samples.

FAM; VIC

5

Infectioushypodermal and Haematopoietic Necrosis Virus

Exogenous

IHHNV

DNA virus

ORF2

Heart, muscle, subcutaneous tissue, gills, hepatopancreas and other tissues; fertilized eggs, juvenile shrimp, young shrimp intact individuals; bait, processed aquatic products and other samples.

FAM; VIC

5

Infectious Muscle Necrosis Virus

Exogenous

IMNV

RNA virus

ORF1

Heart, muscle, subcutaneous tissue, gills, hepatopancreas and other tissues; fertilized eggs, juvenile shrimp, young shrimp intact individuals; bait, processed aquatic products and other samples.

FAM; VIC

1

Yellow Head Virus

Exogenous

YHV

RNA virus

ORF2

Heart, muscle, subcutaneous tissue, gills, hepatopancreas and other tissues; fertilized eggs, juvenile shrimp, young shrimp intact individuals; bait, processed aquatic products and other samples.

FAM; VIC

5

Monodon Baculovirus

Exogenous

MBV

DNA virus

hypothetical protein

Heart, muscle, subcutaneous tissue, gills, hepatopancreas and other tissues; fertilized eggs, juvenile shrimp, young shrimp intact individuals; bait, processed aquatic products and other samples.

FAM; VIC

2.5

Singapore Grouper Iridovirus

Exogenous

SGIV

DNA virus

MCP gene

Fish brain, liver, kidney, spleen, gills and other tissues; bait, processed aquatic products and other samples.

FAM; VIC

5

Carp Spring Viraemia Virus

Exogenous

SVCV

RNA virus

G protein

Fish brain, liver, kidney, spleen, gills and other tissues; bait, processed aquatic products and other samples.

FAM; VIC

5

Covert Mortality Nodavirus

Exogenous

CMNV

RNA virus

RdRp

Heart, muscle, subcutaneous tissue, gills, hepatopancreas and other tissues; fertilized eggs, juvenile shrimp, young shrimp intact individuals; bait, processed aquatic products and other samples.

FAM; VIC

2.5

Macrobrachium Rosenbergii Nodavirus

Exogenous

MrNV

RNA virus

CAP capsid protein gene

Heart, muscle, subcutaneous tissue, gills, hepatopancreas and other tissues; fertilized eggs, juvenile shrimp, young shrimp intact individuals; bait, processed aquatic products and other samples.

FAM; VIC

2.5

Red Seabream Iridovirus

Exogenous

RSIV

DNA virus

DNA polymerase gene

According to the provisions of GB/T18088, and the body length of ≤ 4cm fry to take the whole fish, body length of 4cm ~ 6cm fish to take the internal organs; body length of more than 6cm fish to take the spleen, kidney, heart, intestines and gills.

FAM; VIC

5

Fish Viral Nervous Necrosis Virus

Exogenous

VNNV

RNA virus

Coat protein

Take the brains and eyes of fish, fry and smaller fry with the whole fish. The number of samples taken in accordance with the provisions of GB/T 18088.

FAM; VIC

5

Carp Pox Virus

Exogenous

CyHV-1

DNA virus

5‘UTR

In accordance with the provisions of GB/T18088, fry with body length ≤ 4 cm should be taken as a whole, and the yolk sac should be removed in the case of fish with yolk sac; fry with 4 cm < body length < 6 cm should have all internal organs removed; fish with body length ≥ 6 cm should have gills, spleen and kidney removed. Mature females should also have their ovarian fluid removed; eggs should have their membranes removed.

FAM; VIC

2.5

Goldfish Hemopoietic Organ Necrosis Disease

Exogenous

GFHNV/CyHV-2

DNA virus

DNA polymerase gene

In accordance with the provisions of GB/T18088, fry with body length ≤ 4 cm should be taken as a whole, and the yolk sac should be removed in the case of fish with yolk sac; fry with 4 cm < body length < 6 cm should have all internal organs removed; fish with body length ≥ 6 cm should have gills, spleen and kidney removed. Mature females should also have their ovarian fluid removed; eggs should have their membranes removed.

FAM; VIC

2.5

Koi Herpesvirus Disease

Exogenous

CNGV/KHV/CyHV-3

DNA virus

TK or Sph

In accordance with the provisions of GB/T18088, fry with body length ≤ 4 cm should be taken as a whole, and the yolk sac should be removed in the case of fish with yolk sac; fry with 4 cm < body length < 6 cm should have all internal organs removed; fish with body length ≥ 6 cm should have gills, spleen and kidney removed. Mature females should also have their ovarian fluid removed; eggs should have their membranes removed.

FAM; VIC

5

Grass Carp Reovirus Type 1

Exogenous

GCRV-1

RNA virus

VP7 gene

In accordance with the provisions of GB/T18088, fry with a body length of ≤ 4 cm are taken as a whole after de-tailing; for fish with a body length of 4 cm ~ 6 cm (inclusive), the brain and internal organs (including kidney) are taken; for fish with a body length of 6 cm, the brain, liver, kidney and spleen are taken.

FAM; VIC

5

Grass Carp Reovirus Type 2

Exogenous

GCRV-2

RNA virus

VP2 gene

In accordance with the provisions of GB/T18088, fry with a body length of ≤ 4 cm are taken as a whole after de-tailing; for fish with a body length of 4 cm ~ 6 cm (inclusive), the brain and internal organs (including kidney) are taken; for fish with a body length of 6 cm, the brain, liver, kidney and spleen are taken.

FAM; VIC

5

Grass Carp Reovirus Type 3

Exogenous

GCRV-3

RNA virus

VP2 gene

In accordance with the provisions of GB/T18088, fry with a body length of ≤ 4 cm are taken as a whole after de-tailing; for fish with a body length of 4 cm ~ 6 cm (inclusive), the brain and internal organs (including kidney) are taken; for fish with a body length of 6 cm, the brain, liver, kidney and spleen are taken.

FAM; VIC

5

Vibrio

Exogenous

Vibrio sp

DNA pathogens

rpoA gene

Adult shrimp gills or epithelium, hepatopancreas, stomach, swimming foot or gait foot and other focal areas; fertilized eggs, juvenile shrimp, young shrimp, intact individuals. Bait, processed aquatic products and other samples.

FAM; VIC

2.5

Vibrio Parahaemolyticus

Exogenous

VP

DNA pathogens

gyrB gene

Adult shrimp gills or epithelium, hepatopancreas, stomach, swimming foot or gait foot and other focal areas; fertilized eggs, juvenile shrimp, young shrimp, intact individuals. Bait, processed aquatic products and other samples.

FAM; VIC

1

Enterocytozoon Hepatopenaei and Acute Hepatopancreatic Necrosis Syndromes Syndromes  Multiplex

NoInternal Control

EHP/EMS

DNA pathogens

Specific conservative gene

1, Fertilized eggs, juvenile shrimp, intact individual shrimp, 2, juvenile to adult shrimp or adult shrimp: head and thorax with the eye stalk, intestinal tissue or feces removed. 3, bait, processed aquatic products and other samples

FAM; VIC

Vibrio Parahaemolyticus and Acute Hepatopancreatic Necrosis Syndromes Multiplex

NoInternal Control

VP/EMS

DNA pathogens

Specific conservative gene

1, Fertilized eggs, juvenile shrimp, intact individual shrimp, 2, juvenile to adult shrimp or adult shrimp: head and thorax with the eye stalk, intestinal tissue or feces removed. 3, bait, processed aquatic products and other samples

FAM; VIC

Vibrio Parahaemolyticus and White Spot Syndrome Virus Multiplex

NoInternal Control

VP/WSSV

DNA pathogens and viruses

Specific conservative gene

1, Fertilized eggs, juvenile shrimp, intact individual shrimp, 2, juvenile to adult shrimp or adult shrimp: head and thorax with the eye stalk, intestinal tissue or feces removed. 3, bait, processed aquatic products and other samples

FAM; VIC

Vibrio Parahaemolyticus and Vibrio Harvey Multiplex

NoInternal Control

VP/VH

DNA pathogens

Specific conservative gene

1, Fertilized eggs, juvenile shrimp, intact individual shrimp, 2, juvenile to adult shrimp or adult shrimp: head and thorax with the eye stalk, intestinal tissue or feces removed. 3, bait, processed aquatic products and other samples

FAM; VIC